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Protocols

 

Gene Expression Methods

Nuclei isolation and nuclear run-on assay

Broccoli (Brassica oleracea) RNA polymerase I holoenzyme purification (PDF file)

Promoter-independent RNA polymerase activity assay (PDF file)

Promoter-dependent in vitro transcription and S1 nuclease protection assay (PDF file)

Transient expression in Arabidopsis protoplasts (PEG method)

 

Chromatin Methods

Chromatin immunoprecipitation and ChIP-chop (PDF file)

Bisulfite-mediated DNA sequencing for mapping methylated cytosine positions (PDF file)

Pulsed-field gel electrophoresis of Arabidopsis chromosomal DNA (PDF file)

Purification of core histones from Broccoli (Brssica oleracea) (PDF File)

 

Cytogenetics and Cell Biology Methods

Chromosome counting in DAPI-stained anther filaments

Olga Pontes' protein immunolocalization and DNA-FISH methods (PDF file)

 

Nucleic Acid Purification

Isolation of genomic DNA and total RNA from same total nucleic acid prep

Single leaf DNA prep for Southern blot analysis of transgenic plants in first generation

 

Antibody methods

Affinity purification of anti-peptide antibodies (PDF file)

 

Agrobacterium tumefaciens methods

Freeze/thaw shock or electroporation of plasmid DNA into Agrobacterium tumefaciens

Agrobacterium tumefaciens plasmid DNA mini-prep